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Tools For Diabetes and Weight Control Research:

Functional Human Intestinal Epithelial Cells

GLP1 Elisa

GLP1 Antibodies

Tissue Dissociation (Islets)


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Functional Human Intestinal Epithelial Cells

Further Information on Human Intestinal Epithelial Cells

Intestine epithelial cells play important functions in the maintenance of overall individual health. In addition to their primary roles in absorbing nutrients and in serving as a physical barrier between the outside world and the inside environment, intestine epithelial cells are crucial in maintaining homeostasis of the mammalian immune and endocrine system in the context of health and disease (1). A failing intestine epithelium can lead to various disorders such as hormonal imbalance, inflammatory bowel disease (IBD), weight gain, and diabetes. Thus insights into the mechanisms of and remedies for those disorders can be gained from in vitro systems offered by the availability of human intestine epithelial cells.

Fig.: Characterization of DV Biologics small intestine cells by RT-PCR. Whole small intestine cells (PB-PD007-F, Lane 2), and small intestine epithelial cells (PB-PD015F, passage 4; Lane 3) express the characteristic markers of stem cells, including PHLDA1, an epithelial stem cell marker (3); of epithelial cells (CK18, cytokeratin 18; CDH1, E-caherin); and of entero-endocrine cells. Lane 1: No RT control. Lane 4: Brain tissue (PB-AN001-F).

Human Glucagon,GC ELISA Kit

This protein is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, CUSABIO Inc.]

Cat#                              Product
PB-CSB-E17869h Human glucagon-like peptide 1 receptor (GLP1R) ELISA kit
PB-CSB-E17870h Human glucagon-like peptide 2 receptor (GLP2R) ELISA kit
PB-CSB-E09207h Human Glucagon,GC ELISA Kit
PB-CSB-EQ027317HU Human glucagon like peptide 2(GLP2) ELISA kit

Other species are available on request

Human Glucagon,GC ELISA Kit Manual


Antigen: Short peptide from human GLP-2 sequence.
Isotype: Rabbit IgG
Species & predicted species cross- reactivity ( ): Human, Mouse, Rat
Applications & Suggested starting dilutions:* WB 1:500 - 1:1000 IP 1:100 IHC n/d ICC n/d FACS n/d
Predicted Molecular Weight of protein: 4 kDa
Specificity/Sensitivity: Detects endogenous GLP-2 polypeptides without cross-reactivity with other family members.

Fig:GLP-1 Antibody specifically reacts with GLP-1 polypeptides (5ng/lane) in Western blot. Bottom: This antibody stains paraffin-embedded human pancreas tissue in immunohistochemical analysis.


Tissue Dissociation

Rodent islets offer a valuable tool to study the biology of intracellular signaling pathways and secretory responses. There is strong motivation to increase the yield of healthy islets from each pancreas to reduce animal costs and increase the the utilization of this critical cells preparation. The method of islet isolation using tissue dissociating enzyme (TDE) preparations is well established but inter-laboratory variations in islet yield and quality from any given rodent strain often limit the extent and feasibility of primary islet studies. This variation is in large part due to lot to lot differences in the ill defined collagenase products that are commonly used for rodent islet isolation.

Further Information on Human Islet Isolation
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